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Boom method (Boom nucleic acid extraction method) is a Solid phase extraction method for isolating nucleic acid from a biological sample. This method is characterized by "Absorbing the nucleic acids (NA) to the Silica beads". ==Overview== Boom method (Boom nucleic acid extraction method) 〔 Boom, et. al; US5234809 (), EP0389063 () and their family patents.〕 〔 R Boom, C J Sol, M M Salimans, C L Jansen, P M Wertheim-van Dillen and J van der Noordaa;"Rapid and simple method for purification of nucleic acids."J. Clin. Microbiol. March 1990 vol. 28 no. 3 495-503 ()〕 〔 (Matti Korpela; US6468810 )〕〔 Technical Notes by Bio-Nobile brand ()〕〔By John Brunstein;"Sample extraction methods: how we obtain DNA and RNA" () 〕 〔https://www.hanc.info/labs/labresources/procedures/ACTGIMPAACT%20Lab%20Manual/Standard%20Roche%20Monitor%20Test,%20Boom%20Extraction.pdf〕 〔http://www.siemens.com/about/pool/de/unser_geschaeft/healthcare/diagnostics/perspectives-assay-performance.pdf〕 〔 (How do SPRI beads work? ) 〕 is a solid phase extraction method for "isolating nucleic acid (NA) 〔 In this article, nucleic acid (NA) is meant both DNA and RNA, both in any possible configuration (double-stranded (ds) /single-stranded (ss) /as a combination thereof, Long/Short, Circular/Liner,...). 〕〔 (DNA Extraction Methods For Large Blood Volumes )〕 from Biological samples. Essential of this method is the use of Silica beads, capable of binding the NA in the presence of a chaotropic substance according to the effect. This method is one of the most widespread 〔〔 method for isolating nucleic acids from biological samples and is known as a simple, rapid, and reliable 〔 method for the small-scale purification of NA from Biological sample. This method is said to have been developed and invented by Willem R. Boom et al. around 1990 .〔 Estimated from priority date of Boom, et. al; US5234809 (), EP0389063 () and their family patents.〕 However, the aforementioned chaotropic effect itself was already known and already reported by Vogelstein and Gillespie 〔 B Vogelstein and D Gillespie;"Preparative and analytical purification of DNA from agarose" PNAS 1979 vol. 76 no. 2 pp.615-619 () 〕〔 The following articles was cited in the "Boom, et. al; US5234809 (), EP0389063 () and their family patents". *B Vogelstein and D Gillespie ; "Preparative and analytical purification of DNA from agarose" PNAS 1979 vol. 76 no. 2 pp.615-619 () 〕 before the development of BOOM method. So the contribute of Boom et.al may be the optimization of the method to complex starting materials.〔 , such as body fluids and other biological starting materials, and provides a short step procedure according to the Boom et. al US5234809.〔 And, little later from the Boom et.al 〔 is filed, similar application such as ,〔(), US5973138 (US5342931 )〕 〔(US5705628 )〕 have also been filed by other parties In narrow sense, the word "Silica" meant SiO2 crystals however, other forms of Silica particles are available. Especially amorphous silicon oxide and glass powder, alkylsilica, aluminum silicate (zeolite), or, activated silica with -NH2, are suitable as nucleic acid binding solid phase material according to this method. Today, the embodyments of Boom method, characterized by "utilizing the magnetic beads (silica beads are magnetic beads)" is widely used. In such method, silica beads are captured by magnetic beads collector, such as Tajima Pipette 〔 Hideji Tajima;US5702950 (), US6331277 (), US 2001/0007770 A1 () and their family patents. See also (). Apparatus and methods on this patent are mainly intended to the immune system. However, alternative embodiment for nucleic acids extraction assay are also mentioned in this patent and procedure of Fig.1 is generally similar to Boom method.〕 〔 Hideji Tajima;US6509193 () Apparatus and methods on this patent are mainly intended to the immune system. However, alternative embodiment for nucleic acids extraction assay are also mentioned in this patent and procedure of Fig.1 is generally similar to Boom method. 〕 〔(Urban Schmitt et.al;US6187270 )〕 , Pick pen(R),〔〔 Quad Pole collector,〔(Paul A. Liberti et.al, US5466574 )〕 ..., and so on. Brief procedure and Basic Principle are described below. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Boom method」の詳細全文を読む スポンサード リンク
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